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Two common types of fragmentation in mass spectrometry are charge site-initiated and radical site-initiated fragmentation. In charge site-initiated fragmentation, a bond directly to the charged atom is broken to afford a new fragment cation and a new fragment radical. In radical site-initiated fragmentation, a bond that is remote (often adjacent) to the radical cation atom is cleaved to form a new fragment ion and radical. The fragmentations can occur either heterolytically or homolytically, and multiple possible fragmentations are often possible from any given parent ion.
common fragmentation mechanisms in mass spectrometry
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