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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches - a 2 minute Preview of the Experimental Protocol

Magdia De Jesus, Sarita Ahlawat, Nicholas J. Mantis
New York State Department of Health, Division of Infectious Diseases;

There is an increasing interest in understanding the immunological functions of specific subpopulations of cells in Peyer's patches (PPs), the primary inductive sites of gut-associated lymphoid tissues. Here we outline parallel protocols for preparing PP single cell preparations for flow cytometric analysis and PP cryosections for immunostaining.

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