The number of treatments in the immuno-oncology pipeline continues to increase. If the treatment is CAR-T cells, adoptive T cell therapy or checkpoint inhibitors, the mechanism of action includes T cell lysis of tumor cells.

Incucyte® Live-Cell Analysis Systems, combined with fluorescent labels of cytotoxicity, offer remote monitoring of tumor cell lysis. This webinar includes discussion on: -- The specific lysis of tumor cells -- Comparing Annexin V and Caspase 3/7, for readout and analysis options -- The use of adherent vs suspension cells as targets.

Watch the full webinar here: [ Ссылка ]

0:16 Live cell imaging: after looking at our alternatives we chose to use the Incucyte S3. Once you set up the imaging of the culture you can walk away and come in the next day and the data is pretty much ready to analyse. That really is attractive us.

0:38 Mart-1 specific T cells on left, CMV specific T cells on the right: “Using that Caspase label does show up a lot more cell death in the T cell population.

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