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Blood Agar Preparation steps
Blood Agar Preparation Methods
Blood agar base
Composition of Sheep Blood Agar Base
Ingredients Gms / Litre
Casein enzymic hydrolysate 14.000
Peptic digest of animal tissue 4.500
Yeast extract 4.500
Sodium chloride 5.000
Agar 12.500
Final pH (at 25°C) 7.3±0.2
Procedure for the Preparation of Blood Agar
Suspend 40.5 grams in 1000 ml distilled water or deionized water.
Heat to boiling to dissolve the medium completely.
Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Cool to 45-50°C and aseptically add 7% sterile sheep blood.
Mix well and pour into sterile Petri plates. Avoid the formation of air bubbles. You must have warmed the blood to room temperature at the time of dispensing to a molten agar base.
Dispense 15 ml amounts to sterile Petri plates aseptically
Label the medium with the date of preparation and give it a batch number (if necessary).
Blood Agar Preparation for Cultivation of Bacteria
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