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An infection can be caused by either gram-positive or gram-negative bacteria. Knowing which type of bacteria that caused the infection will help determine the right antibiotic for each infection. We would not want to just give a broad-spectrum antibiotic randomly because that would contribute to creating superbugs. The zone of inhibition in the antibiotic sensitivity test will help determine which antibiotic works for each type of bacteria.
Timestamps:
0:59 – Overview
1:48 – Why do we have to prepare an inoculum?
2:33 - How to prepare McFarland turbidity standards?
5:04 - Disk diffusion method or Kirby-Bauer method
7:00 - A Mueller-Hinton agar plate
8:15 – Disk diffusion method procedure and interpretation
13:56 – Example
15:50 – Blood Talks Fun Times: trouble shoot
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Welcome to Blood Talks Fam. Blood Talks offers you a peek into how clinical laboratory scientists (CLS) or medical laboratory technologists (MLT) work in a hospital. CLS/MLT hold an important role in the healthcare system, but unfortunately, many people do not know that they even exist, simply because most of CLS/MLT work is done behind the scenes. In this channel, you can find explanations from a clinical laboratory point of view. I hope you enjoy and learn something you did not know before.
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As always: Remember, your blood tells you the story of your health.
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Disclaimer
Information provided here is for education and entertainment purposes only, not intended for diagnosis. Please consult your doctor for any health related issues you may have. As new medical technologies are updated, there are more ways to cure illnesses, new testing methods and the older methods could be obsolete. Therefore, please double check your information for the most up-to-date facts.
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Tags
Blood Bank, Blood Talk, Blood Talks, Transfusion Medicine, clinical laboratory scientists, medical laboratory technologists, healthcare worker ,healthcare professions, microbiology, gram positive bacteria, gram negative bacteria, cell wall, antibiotic sensitivity test, antibiotic susceptibility, zone of inhibition, antibiotic disk, supper bugs, resistant, intermediate, susceptible, Kirby-Bauer method, disc diffusion method, inhibition zone, McFarland standard, immunohematology
