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Pour plate method is usually the method of choice for counting the number of colony-forming bacteria present in a liquid specimen. In this method, a fixed amount of inoculum (generally 1 ml) from a broth/sample is placed in the centre of sterile Petri dish using a sterile pipette. Molten cooled agar (approx. 15mL) is then poured into the Petri dish containing the inoculum and mixed well. After the solidification of the agar, the plate is inverted and incubated at 37°C for 24-48 hours.
Microorganisms will grow both on the surface and within the medium. Colonies that grow within the medium generally are small in size and maybe confluent; the few that grow on the agar surface are of the same size and appearance as those on a streak plate. Each (both large and small) colony is carefully counted (using magnifying colony counter if needed). Each colony represents a “colony-forming unit” (CFU).
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