For more information, visit [ Ссылка ] This video shows an adult mouse brain hemisphere that was subjected to our tissue clearing protocol using the MACS® Clearing Kit. Here, you see the last step of the protocol which renders the sample transparent for volumetric fluorescence imaging using light sheet microscopy. During this step, the refractive index of the sample is matched to the refractive index of the MACS Imaging Solution (1.556–1.562) within only 6 hours.

The MACS® Clearing Kit & MACS® Imaging Solution are non-toxic organic tissue clearing reagents and part of Miltenyi Biotec’s 3D imaging workflow comprising easy-to-follow protocols, antibodies validated for 3D-immunofluorescence, and the fully automated light sheet microscope UltraMicroscope Blaze™.

Miltenyi Biotec is a global provider of products and services that advance biomedical research and cellular therapy. Our innovative tools support research at every level, from basic research to translational research to clinical application. This integrated portfolio enables scientists and clinicians to obtain, analyze, and utilize the cell. Our technologies cover techniques of sample preparation, cell isolation, cell sorting, flow cytometry, cell culture, molecular analysis, and preclinical imaging. Our 30 years of expertise spans research areas including immunology, stem cell biology, neuroscience, and cancer, and clinical research areas like hematology, graft engineering, and apheresis. In our commitment to the scientific community, we also offer comprehensive scientific support, consultation, and expert training. Today, Miltenyi Biotec has 3,000 employees in 28 countries – all dedicated to helping researchers and clinicians around the world make a greater impact on science and health.

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