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Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition - a 2 minute Preview of the Experimental Protocol
Sanggil Kim, Wooseok Ko, Hyunji Park, Hyun Soo Lee
Sogang University, Department of Chemistry;
Here, we present a protocol to site-specifically introduce chemical probes into an antibody fragment by genetically incorporating an azide-containing amino acid, and subsequently coupling the azide with a chemical probe by strain-promoted azide-alkyne cycloaddition (SPAAC).
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