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Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs) - a 2 minute Preview of the Experimental Protocol

Johanna E. Kowalko, Li Ma, William R. Jeffery
Iowa State University, Genetics, Development and Cell Biology; University of Cincinnati, Department of Biological Sciences; University of Maryland, Department of Biology;

Gene-targeting mutagenesis is now possible in a wide range of organisms using genome editing techniques. Here, we demonstrate a protocol for targeted gene mutagenesis using transcription activator like effector nucleases (TALENs) in Astyanax mexicanus, a species of fish that includes surface fish and cavefish.

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