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Development of automated imaging and analysis for zebrafish chemical screens. - a 2 minute Preview of the Experimental Protocol

Andreas Vogt, Hiba Codore, Billy W. Day, Neil A. Hukriede, Michael Tsang
University of Pittsburgh Drug Discovery Institute, Pharmacology and Chemical Biology; University of Pittsburgh, Department of Microbiology and Molecular Genetics; University of Pittsburgh, Department of Pharmaceutical Sciences; University of Pittsburgh, Department of Chemistry;

We report the development of a system for automated imaging and analysis of zebrafish transgenic embryos in multiwell plates. This demonstrates the ability to measure dose dependent effects of a small molecule, BCI, on Fibroblast Growth Factor reporter gene expression and provide technology for establishing high-throughput zebrafish chemical screens.

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