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Measuring Fast Calcium Fluxes in Cardiomyocytes - a 2 minute Preview of the Experimental Protocol
Urszula Golebiewska, Suzanne Scarlata
Queensborough Community College, Department of Biological Sciences and Geology; Stony Brook University, Department of Physiology & Biophysics;
We present a method to isolate rapid (microsecond) calcium events from slower fluxes in living cells using laser scanning confocal microscopy. The method measures fluorescence intensity fluctuations of calcium indicators by recording line scans of several hundred pixels in a cell. Histogram analysis allows us to isolate the time scales of different calcium fluxes.
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