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3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer - a 2 minute Preview of the Experimental Protocol
Yin Wang, Mark L. Day, Diane M. Simeone, Phillip L. Palmbos
University of Michigan Medical Center, Departments of Internal Medicine, Hematology/Oncology Division, Rogel Cancer Center; University of Michigan Medical Center, Department of Urology, Division of GU Oncology, Rogel Cancer Center; NYU Langone Health, Departments of Surgery and Pathology, Perlmutter Cancer Center;
The processes governing bladder cancer invasion represent opportunities for biomarker and therapeutic development. Here we present a bladder cancer invasion model which incorporates 3-D culture of tumor spheroids, time-lapse imaging and confocal microscopy. This technique is useful for defining the features of the invasive process and for screening therapeutic agents.
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