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Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos - a 2 minute Preview of the Experimental Protocol

Lorena Benedetti, Anna Ghilardi, Laura Prosperi, Maura Francolini, Luca Del Giacco
Università degli Studi di Milano, Department of Medical Biotechnology and Translational Medicine; Howard Hughes Medical Institute, Yale University School of Medicine, Department of Neuroscience; Department of Cell Biology; Yale University School of Medicine, Program in Cellular Neuroscience, Neurodegeneration and Repair; Università degli Studi di Milano, Department of BioSciences;

Protocols described here allow for the study of the electrical properties of excitable cells in the most non-invasive physiological conditions by employing zebrafish embryos in an in vivo system together with a fluorescence resonance energy transfer (FRET)-based genetically encoded voltage indicator (GEVI) selectively expressed in the cell type of interest.

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