This video shows how to prepare sterile media for use in tissue culture. Watch our scientists as they walk through the basic steps of media preparations and sterile filtration. With this video, you will learn:
• The role of media in tissue culture
• How media helps to create a physiological environment for your cells
• The components of basic basal media and complex media, and when to use each type
• The different types of media supplements, including serums, such as fetal bovine serum (FBS), amino acids, and antibiotics
• When to sterile filter your complete media
Media preparation in cell culture can significantly affect both mammalian cell growth and experimental outcome. Tissue culture conditions can vary depending on the cell type, but all cells in culture require:
• A sterile environment
• Specific handling techniques
• A steady supply of nutrients.
Learn more about the types of media and which supplements to add for your projects.
For more information about cell culture media preparation, please visit:
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