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Assaying Protein Kinase Activity with Radiolabeled ATP - a 2 minute Preview of the Experimental Protocol

Aroon S. Karra, Steve Stippec, Melanie H. Cobb
University of Texas Southwestern Medical Center, Department of Pharmacology;

Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.

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