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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation - a 2 minute Preview of the Experimental Protocol

Ryan C. Lee, Travis R. Douglas, Leo Y. T. Chou
University of Toronto, Institute of Biomedical Engineering;

We describe the engineering of a novel DNA-tethered T7 RNA polymerase to regulate in vitro transcription reactions. We discuss the steps for protein synthesis and characterization, validate proof-of-concept transcriptional regulation, and discuss its applications in molecular computing, diagnostics, and molecular information processing.

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