New methods to improve super-resolution imaging techniques are giving biologists a clearer and more complete view of the inner workings of living cells. Read more at [ Ссылка ]
In this video: A new paper in Nature Biotechnology from the Shroff Lab details two practical ways to improve the axial, or z, resolution of 3D structured illumination microscopy, a technique to see inside living cells. In one method, a mirror is added to the microscope to create an additional beam of light, changing the interference pattern, and enabling finer, sharper resolution along the z axis. (video 1). The second method, which uses deep learning, blurs the sharp x and y axes to look like the blurry z axis and then trains a neural network to reverse these blurry images. The network then uses that information to un-blur the z axis. (video 2).
Video Credit: Li et al.
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