This video is about :
Van den bergh reaction
its importance in the diagnosis of jaundice.
excretory function of liver
types of bilirubin
importance of van den bergh reaction
mechanism of reaction
principle of reaction
composition of reagent
direct bilirubin
indirect bilirubin
total bilirubin
The Van den Bergh test, also known as the Van den Bergh reaction or bilirubin test, is a diagnostic test used to assess liver function and detect the presence of jaundice, a condition characterized by the yellowing of the skin and eyes due to elevated levels of bilirubin in the blood. The test is named after the Dutch biochemist Dirk Van den Bergh, who developed the method.
The Van den Bergh test measures the level of conjugated (direct) and unconjugated (indirect) bilirubin in the blood. Bilirubin is a yellow pigment produced as a result of the breakdown of heme, a component of hemoglobin in red blood cells. It is primarily processed and eliminated by the liver.
Here's how the Van den Bergh test is typically performed:
1. Blood sample collection: A blood sample is collected from the patient, usually through a vein in the arm. The blood is collected into a special tube containing an anticoagulant to prevent clotting.
2. Serum separation: The blood sample is then centrifuged to separate the serum, which is the clear liquid portion of the blood, from the cellular components.
3. Reaction with diazo reagent: The serum is mixed with a diazo reagent, which is a solution containing a strong acid and a diazonium salt. The diazo reagent reacts with bilirubin to form a colored product.
4. Measurement of color intensity: The intensity of the color formed is measured spectrophotometrically, typically at a wavelength of 540 nm. The intensity of the color is proportional to the concentration of bilirubin in the sample.
Based on the color intensity, the Van den Bergh test allows for the determination of the total bilirubin level in the blood. In addition, the test can differentiate between conjugated (direct) and unconjugated (indirect) bilirubin by adding a solubilizing agent, such as alcohol, to the reaction mixture. Conjugated bilirubin remains in solution, while unconjugated bilirubin precipitates and is measured separately.
The results of the Van den Bergh test provide valuable information about liver function and the presence of jaundice. Elevated levels of total bilirubin, especially an increased proportion of conjugated bilirubin, may indicate liver dysfunction, biliary obstruction, or other conditions affecting the normal processing and elimination of bilirubin.
It's important to note that the Van den Bergh test is just one of several tests used to evaluate liver function and diagnose jaundice. Additional tests, such as liver function panel, ultrasound, and imaging studies, may be required to determine the underlying cause of jaundice and guide appropriate treatment. Medical professionals and healthcare providers are best suited to interpret the results of the Van den Bergh test in the context of the patient's overall clinical condition.
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#directbilirubin
#indirectbilirubin
#conjugatedbilirubin
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