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Pyrosequencing for Microbial Identification and Characterization - a 2 minute Preview of the Experimental Protocol
Patrick J. Cummings, Ray Ahmed, Jeffrey A. Durocher, Adam Jessen, Tamar Vardi, Kristina M. Obom
Johns Hopkins University, Center for Biotechnology Education, Krieger School of Arts and Sciences; Qiagen Sciences, Inc.,;
Pyrosequencing is a versatile technique that facilitates microbial genome sequencing that can be used to identify bacterial species, discriminate bacterial strains, and detect genetic mutations that confer resistance to anti-microbial agents. In this video, the procedure for microbial amplicon generation, amplicon pyrosequencing, and DNA sequence analysis will be demonstrated.
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