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An Efficient Method for Directed Hepatocyte-Like Cell Induction from Human Embryonic Stem Cells - a 2 minute Preview of the Experimental Protocol
Qi Zhou, Xiaoling Xie, Zhiqian Zhong, Pingnan Sun, Xiaoling Zhou
Shantou University Medical College, Stem Cell Research Center; Shantou University Medical College, The Center for Reproductive Medicine; Shantou University Medical College, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology;
This manuscript describes a detailed protocol for differentiation of human embryonic stem cells (hESCs) into functional hepatocyte-like cells (HLCs) by continuously supplementing Activin A and CHIR99021 during hESC differentiation into definitive endoderm (DE).
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