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Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation - a 2 minute Preview of the Experimental Protocol
Arun Sharma, Guang Li, Kuppusamy Rajarajan, Ryoko Hamaguchi, Paul W. Burridge, Sean M. Wu
Stanford University School of Medicine, Stanford Cardiovascular Institute; Stanford University School of Medicine, Institute of Stem Cell Biology and Regenerative Medicine, Cardiovascular Medicine Division, Department of Medicine, Child Health Research Institute;
Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.
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