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Imaging Glycans in Zebrafish Embryos by Metabolic Labeling and Bioorthogonal Click Chemistry - a 2 minute Preview of the Experimental Protocol

Hao Jiang, Lei Feng, David Soriano del Amo, Ronald D. Seidel III, Florence Marlow, Peng Wu
Albert Einstein College of Medicine, Yeshiva University, Department of Biochemistry; Albert Einstein College of Medicine, Yeshiva University, Macromolecular Therapeutics Development Facility; Albert Einstein College of Medicine, Yeshiva University, Developmental and Molecular Biology;

A click-chemistry based method that allows for the rapid, noninvasive, and robust labeling of alkyne-tagged glycans in zebrafish embryos is described. Fucosylated glycans in the enveloping layer of zebrafish embryos in the late gastrulation stage were imaged in this study.

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