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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis - a 2 minute Preview of the Experimental Protocol

Elizabeth R. Zielins, Ruth Tevlin, Michael S. Hu, Michael T. Chung, Adrian McArdle, Kevin J. Paik, David Atashroo, Christopher R. Duldulao, Anna Luan, Kshemendra Senarath-Yapa, Graham G. Walmsley, Taylor Wearda, Michael T. Longaker, Derrick C. Wan
Stanford University School of Medicine, Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery; Stanford University, Institute for Stem Cell Biology and Regenerative Medicine;

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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