In this video, we explore the different types of columns used in High Performance Liquid Chromatography (HPLC) and the analytes they are best suited to separate.

Reverse phase columns are ideal for hydrophobic compounds, normal phase columns for polar compounds, ion-exchange columns for inorganic ions, amino acids, and peptides, and size-exclusion columns for macromolecules. By understanding the different types of columns and their ideal uses, you can optimize your HPLC system to produce reliable analytical results.

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