Learn how to use FIJI (ImageJ) to create 3-dimensional (3D) models from 2D image stacks. The plugin called 3D script is used for reconstruction and rendering. A step-by-step procedure for creating simple animations is also shown. This video combines 2 previously released (2021) videos:
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Plugin credit:
Schmid, B.; Tripal, P. & Fraaß, T. et al. (2019), "3Dscript: animating 3D/4D microscopy data using a natural-language-based syntax", Nature methods 16(4): 278-280, PMID 30886414.
z stack image credit on animation portion:
Nicole Szeluga, M. Frank lab, Cornell University
- the z stack of a wild-type anther was acquired with a 40x/1.2 water immersion objective lens on a Zeiss LSM 880 confocal microscope.
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