Watch the Full Video at [ Ссылка ].
Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning - a 2 minute Preview of the Experimental Protocol
Jonghee Yoon, YoungJu Jo, Young Seo Kim, Yeongjin Yu, Jiyeon Park, Sumin Lee, Wei Sun Park, YongKeun Park
University of Cambridge, Department of Physics; Korea Advanced Institute of Science and Technology, Department of Physics; Korea Advanced Institute of Science and Technology, KAIST Institute for Health Science and Technology; Tomocube, Inc.,; Korea Advanced Institute of Science and Technology, Department of Chemical and Biomolecular Engineering; Korea Advanced Institute of Science and Technology, Department of Biological Sciences; Stanford University, Department of Applied Physics;
We describe a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and a machine learning algorithm. Measurements of 3D refractive index tomograms of lymphocytes present 3D morphological and biochemical information for individual cells, which is then analyzed with a machine-learning algorithm for identification of cell types.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
