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In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers - a 2 minute Preview of the Experimental Protocol
Beatrice Ramm, Philipp Glock, Petra Schwille
Max Planck Institute of Biochemistry, Department of Cellular and Molecular Biophysics;
We provide a protocol for in vitro self-organization assays of MinD and MinE on a supported lipid bilayer in an open chamber. Additionally, we describe how to enclose the assay in lipid-clad PDMS microcompartments to mimic in vivo conditions by reaction confinement.
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