Chapter 08 Microbial Genetics and Genetic Engineering - Cowan - Dr. Mark Jolley

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Learning Outcomes: Section 8.1 – Approximately 47 minutes
1. Define the terms genome and gene.
2. Differentiate between genotype and phenotype.
3. Draw a picture of a length of DNA, including all important chemical groups.
4. Explain how DNA replication takes place.
5. Use Okazaki fragments to explain leading and lagging strands.
0:48 - 3:08:47


Learning Outcomes: Section 8.2 – Approximately 48 minutes
6. Relate the new and old versions of the "central dogma.”
7. Identify important differences between RNA and DNA.
8. Draw a picture of the process of transcription.
9. List three types of RNA directly involved in translation.
10. Define codon and anticodon.
11. Identify on which molecules the promoter, start codon, and the A and P sites appear.
12. Indicate how eukaryotic transcription and translation differ from these processes in prokaryotes.
47:41 - 3:08:47


Learning Outcomes: Section 8.3 - Approximately 20 minutes
13. List one or two important advantages to arranging genes in an operon.
14. Highlight the main points of lac operon operation.
1:35:34 - 3:08:47

Learning Outcomes: Section 8.4 - Approximately 20 minutes
15. Define recombinant.
16. Describe three forms of horizontal gene transfer used in bacteria.
1:51:15 - 3:08:47

Learning Outcomes: Section 8.5 - Approximately 20 minutes
17. Define the term mutation and discuss its importance.
18. Differentiate among frameshift, nonsense, silent, and missense mutations.
2:20:07 - 3:08:47

Learning Outcomes: Section 8.6 - Approximately 20 minutes
19. Explain the importance of restriction endonucleases to genetic engineering
20. List the steps in the polymerase chain reaction.
21. Describe how you can clone a gene into a bacterium.
2:41:36 - 3:08:47

Dr. Mark Jolley, Cowan, Microbiology, Eagle Gate College, Provo College, Microbiology Fundamentals,

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