The ESR Facility addresses research at the interface of free radical biology and medicine. We provide the expertise and instrumentation to pursue research questions dealing with oxygen free radicals, singlet oxygen, nitric oxide and the array of related oxidants and antioxidants that influence the overall redox environment of cells, tissues, and whole organisms. Our goal is to advance the fundamental understanding of free radical biology to improve human health through innovative approaches in the prevention and treatment of disease. Example services include: 1) EPR spectroscopy to detect free radicals, directly or with spin trapping approaches or redox active transition metals, e.g. Fe3+ and Cu2+; 2) Sievers NOA280i for the detection of footprints of nitric oxide, e.g. nitrite and nitrate; 3) Clark Electrode for the detection of oxygen uptake by chemical, biochemical, and cellular systems; 4) Seahorse XF96 to quantitatively determine (and dissect) rates of production of ATP by cells; 5) Quantitative assays for a wide range of species, e.g. vitamin C, superoxide, GPx4, and other species of interest in the field of redox biology.

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