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FEHLING REAGENT
In this test the presence of aldehydes but not ketones is detected by reduction of the deep blue solution of copper(II) to a red precipitate of insoluble copper oxide. The test is commonly used for reducing sugars but is known to be NOT specific for aldehydes. For example, fructose gives a positive test with Fehling's solution as does acetoin. Fehling's solution is the name given to a chemical reagent, primarily used to differentiate between water-soluble carbohydrate and ketone functional groups. It can also be used as a test for reducing sugars and non-reducing sugars. This reagent is prepared from two solutions, a basic solution of potassium sodium tartrate and a solution of copper(II) sulfate.
Fehling’s test is one of the sensitive test for detection of reducing sugars. Fehling’s reagents comprises of two solution Fehling’s solution A and solution B. Fehling’s solution A is aqueous copper sulphate and Fehling’s solution B is alkaline sodium potassium tartrate ( Rochelle salt). Rochelle salts (sodium potassium tartrate) present in the reagent acts as the chelating agent in this reaction. These two solutions are mixed in equal amount before the test.
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