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Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations - a 2 minute Preview of the Experimental Protocol
Alec Kittredge, Changyi Ji, Yu Zhang , Tingting Yang
University of Rochester, School of Medicine and Dentistry, Department of Pharmacology and Physiology;
Here we present a protocol to differentiate retinal pigment epithelium (RPE) cells from human pluripotent stem cells bearing patient-derived mutations. The mutant cell lines may be used for functional analyses including immunoblotting, immunofluorescence, and patch clamp. This disease-in-a-dish approach circumvents the difficulty of obtaining native human RPE cells.
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