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Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens - a 2 minute Preview of the Experimental Protocol

Erica L. Benard, Astrid M. van der Sar, Felix Ellett, Graham J. Lieschke, Herman P. Spaink, Annemarie H. Meijer
Leiden University, Department of Molecular Cell Biology, Institute of Biology; VU University Medical Center, Department of Medical Microbiology and Infection Control; Monash University, Australian Regenerative Medicine Institute;

Transparent zebrafish embryos have proved useful model hosts to visualize and functionally study interactions between innate immune cells and intracellular bacterial pathogens, such as Salmonella typhimurium and Mycobacterium marinum. Micro-injection of bacteria and multi-color fluorescence imaging are essential techniques involved in the application of zebrafish embryo infection models.

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