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Efficient and Cost Effective Electroporation Method to Study Primary Cilium-Dependent Signaling Pathways in the Granule Cell Precursor - a 2 minute Preview of the Experimental Protocol
Jason Cho Wai Lo, Wee Lin Wong, Catherine Hong Huan Hor
Hong Kong Baptist University, Department of Chemistry, Faculty of Science;
Here, we present a reproducible in vitro electroporation protocol for genetic manipulation of primary cerebellar granule cell precursors (GCPs) that is cost-effective, efficient, and viable. Moreover, this protocol also demonstrates a straightforward method for the molecular study of primary cilium-dependent Hedgehog signaling pathways in primary GCP cells.
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