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Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut - a 2 minute Preview of the Experimental Protocol

Toru Kariu, Adam S. Coleman, John F. Anderson, Utpal Pal
University of Maryland, Department of Veterinary Medicine; Connecticut Agricultural Experiment Station, Department of Entomology;

Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.

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