There's a total of 40 Prayer Beads in Sekiro Shadows Die Twice. 4 Prayer Beads can be combined into Prayer Necklaces, which will increases to your Posture and Vitality. Collecting all 40 will unlock the Peak Physical Strength Trophy / Achievement.
Ashina Outskirts
(0:01) 1/40 General Naomori Kawarada - Outskirts Wall – Gate Path
(0:40) 2/40 Chained Ogre - Outskirts Wall – Stairway
(1:08) 3/40 General Tenzen Yamauchi - Outskirts Wall – Stairway
(1:44) 4/40 Treasure Chest - Ashina Castle Gate
(2:57) 5/40 Blazing Bull - Ashina Castle Gate
Hirata Estate
(4:09) 6/40 Shinobi Hunter Enshin of Misen - Estate Path
(5:22) 7/40 Juzou the Drunkard - Main Hall
(6:25) 8/40 Treasure Chest - Audience Chamber
Ashina Castle
WARNING!!! Complete the following before defeating the Corrupted Monk & Guardian Ape
(7:10) 9/40 General Kuranosuke Matsumoto - Ashina Castle
(7:34) 10/40 Merchant $1400 - Abandoned Dungeon Entrance
(8:24) 11/40 Lone Shadow Longswordman - Ashina Reservoir
(9:16) 12/40 Seven Achina Spears – Shikibu Toshikatsu Yamauchi - Ashina Reservoir
(10:55) 13/40 Treasure Chest - Upper Tower – Antechamber
(11:29) 14/40 Ashina Elite Jinsuke Saze - Upper Tower – Ashina Dojo
Senpou Temple, Mt. Kongo
(11:55) 15/40 Armored Warrior - Shugendo
(13:10) 16/40 Long-arm Gentipede Sen’un - Temple Grounds
(14:03) 17/40 Treasure Chest - Temple Grounds (After you defeated the Corrupted Monk you will get the ability to swim underwater)
Ashina Depths
(14:48) 18/40 Snake Eyes Shirahagi - Ashina Depths
(15:27) 19/40 On Top of the Statue Head - Ashina Depths
(16:16) 20/40 Tokujiro the Glutton - Hidden Forest
(17:41) 21/40 O’Rin of the Water - Watermill
(18:12) 22/40 Treasure Chest - Wedding Cave Door
(18:58) 23/40 Treasure Chest Underwater - Mibu Village (Defeat the Corrupted Monk first)
Sunken Valley
(19:41) 24/40 Item Pickup - Under-Shrine Valley
(20:30) 25/40 Snake Eyes Shirafuji - Gun Fort
(21:40) 26/40 Long-arm Gentipede Giraffe - Gun Fort
(22:04) 27/40 Item Pickup - Gun Fort
PLEASE NOTE!!! Make sure you collect all 27 of these above before defeating the Corrupted Monk & Guardian Ape
(22:58) PLEASE NOTE!!! When you talk to the Owl, Pick the 2nd dialogue option “Break the Iron Code, Stay Loyal to Kuro”. After you defeat the Owl pick up the next 5 before you complete the Fountainhead Palace
(23:03) 28/40 Lone Shadow Vilehand - Ashina Castle – Upper Tower Dojo
(23:34) 29/40 & 30/40 Undying Guardian Ape and normal Guardian Ape - Ashina Depths
(24:36) 31/40 Chained Ogre - Ashina Castle – Upper Tower Antechamber
(25:28) 32/40 Lone Shadow Masanaga the Spear-Bearer - Ashina Castle – Old Grave
PLEASE NOTE!!! The next area is the Fountainhead Palace. When you talk to the Owl, pick the 2nd dialogue option “Break the Iron Code, Stay Loyal to Kuro”. This will give you access to the Fountainhead Palace.
PLEASE NOTE!!! PART-2 You need to start the Purification Ending.
1. (26:27) After defeating Owl talk to Kuro and exhaust all dialogues. You will light an incense burner and receive "Sweet Rice Ball"
2. (26:32) Go around the corner and Eavesdrop on Kuro then rest
3. (26:37) Head upstairs to talk to Emma and agree with her then rest.
4. (26:42) Go to upstair and talk to Emma again and receive "Tomoe's Note" then rest.
5. (26:47) Go to the Old Grave Idol and talk to Emma by the graves to the left.
6. (26:52) Go to the Dilapidated Temple Idol and Eavesdrop on the Sculptor and Emma
7. (26:57) Rest then talk Emma until she gives you a Bell to use at the Buddha statue. This will take you to Hirata Estate.
Hirata Estate Purification Ending
(27:02) 33/40 Lone Shadow Masanaga the Spear-Bearer - Hirata Estate Main Hall
(27:54) 34/40 Juzou the Drunkard - Main Hall
Fountainhead Palace
(29:08) 35/40 Sakura Bull - Flower Viewing Stage
(30:06) 36/40 Okami Leader Shizu - Great Sakura
(30:29) 37/40 Treasure Chest Underwater - Feeding Ground (Defeated the Great Colored Carp to access it)
PLEASE NOTE!!! Available after defeating the Divine Dragon
(31:55) 38/40 Ujinari Mizou - Ashina Castle – Upper Tower Dojo
(32:36) 39/40 Shigekichi of the Red Guard - Ashina Castle – Old Grave
(33:31) 40/40 Seven Spears Ashina – Shume Masaji Oniwa - Ashina Castle – Ashina Reservoir
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