Learn how to calculate genetic transformation efficiency with this whiteboard video.
** Note, in this video we reference a "second dilution" the second dilution being referenced isn't a formal dilution. Instead, it is the plating of the cells. For ease, it's been referred to as Dilution #2 or a second dilution**
Transformation efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid into a given volume of competent cells.
TE = Colonies/μg/Dilution
Where:
Colonies = the number of colonies counted
μg = amount of DNA transformed in μg
Dilution = total dilution of the DNA before plating
Transformation Efficiency Calculator - [ Ссылка ]
Products:
GB10B Chemically Competent E. coli Cells: [ Ссылка ]
GoldBio's Competent Cells - [ Ссылка ]
Related articles/resources:
Introduction to Competent Cells - [ Ссылка ]
A Quick Overview of Molecular Cloning - [ Ссылка ]
DH10B Chemically Competent E. coli Cells Transformation Protocol - [ Ссылка ]
GoldBio's Competent Cell Guide - [ Ссылка ]
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