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Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue - a 2 minute Preview of the Experimental Protocol

Jie Lu, Laurent C. Delli-Bovi, Jonathan Hecht, Rebecca Folkerth, Volney L. Sheen
Beth Israel Deaconess Medical Center, Department of Neurology; Brigham and Women's Hospital, Department of Obstetrics and Gynecology; Beth Israel Deaconess Medical Center, Department of Pathology; Brigham and Women's Hospital, Department of Pathology, Division of Neuropathology;

A simple and reliable method on isolation and culture of neural stem cells from discarded human fetal cortical tissue is described. Cultures derived from known human neurological disorders can be used for characterization of pathological cellular and molecular processes, as well as provide a platform to assess pharmacological efficacy.

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