Lecture explaining Antisense RNA technology-
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The application of Antisense RNA technology is in many sectors. This technology was used in Flavr Savr, for tomato ripening; ripening in tomato produces enzyme Polygalactourodase (PG) which softens tomatoes and finally rotten them quickly. Two biotechnology companies: Calgene, USA and ICI Seeds, UK introduced a gene in plant which synthesizes a complementary mRNA to PG gene and inhibites the synthesis of PG enzyme delaying over ripening and rotting. Antisense therapy is considered for treating certain genetic disorders and infections. This is also referred to as Gene Therapy. It includes: isolation of specific gene; it's cloning and inserting it into target tissue cells to make desired protein. It has to be ensured that the gene is expressed correctly and sufficiently without causing harm to patient in context with the immune response. Antisense RNA technology is also used for cancer therapy. This therapy is used for treating brain cancer -- malignant glioma and cancer of prostate gland, in malignant glioma IGF-I was over produced and in the prostate cancer IGF-IR was synthesized more. These two were used to block the translation. Research is carried on in regard with Antisense RNA drugs for treatment of CMV, HIV, cancer, etc. Antisense antiviral drug named Formivirsen is developed to treat CMV, which was licensed by FDA in August 1998. In 2010, scientist at NIPGR by using Antisense technology developed tomato which could last longer for more than 30 days by silencing two genes (alpha - man and beta -- hex) which causes softening and wrinkling in tomatoes during ripening process. Source of the article published in description is Wikipedia. I am sharing their material. © by original content developers of Wikipedia.
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