How to Prepare Blood Agar | Media in Microbiology Lab | Media for Hemolysis Detection | Basic Science Series
Tryptic Soy Agar (Blood Agar Base),
Pancreatic digest of casein (mammalian milk protein) 15.0 g
Papaic digest of soy meal 5.0 g
NaCl 5.0 g
Agar 15.0 g
“sterilize by autoclaving”
Cool media to 45 to 50°C, warm blood
add 5% (vol/vol) sterile defibrinated blood
Mix thoroughly + Avoid bubbles + Carefully pour in plates
General-purpose enriched medium
Differentiate bacteria (Hemolysis)
Human blood is discouraged (human blood-borne pathogens)
Blood Agar Is Not a Selective Medium!
Blood Agar" is not a consistently defined medium!
Ensure safety as per the standard lab safety protocols

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Key words
Blood agar base, Bacteriology, Microbiology lab experiments, Microbiology lectures, Microbes growth media, Blood agar media, How to prepare media for bacteria, how to grow bacteria in lab, blood agar media preparation, Enriched media, Hemolysis media, Tryptic Soy Agar, how to mix media and blood to make blood agar, media to grow pathogenic bacteria, defibrinated blood.

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