Use of the CHEMetrics K-7433 Vacu-vials® instrumental test kit and our A-0214 snapper allows analysts to test for ozone in bottled water up to 0.75 ppm.

INSTRUCTIONS
First, a reagent blank absorbance value must be obtained for each ampoule prior to using it to perform the test procedure below. Insert the unsnapped ampoule into the instrument cell holder and obtain an absorbance value for the reagent. CHEMetrics pre-programmed photometers will retain this reagent blank value. If using a spectrophotometer, record the value for later use. This is the Unsnapped Absorbance. Proceed with the Test Procedure using this ampoule.

TEST PROCEDURE
1. Fill the sample cup to the 25 mL mark with the sample to be tested, being careful to minimize turbulence. NOTE: Ozone loss from sample occurs rapidly. Do not transfer sample to other containers.
2. Immediately place the Vacu-vials ampoule from which the reagent blank value was generated, tip first, into the sample cup. Snap the tip. The ampoule will fill leaving a bubble for mixing.
3. Invert the ampoule continuously for a full **30 seconds**, allowing the bubble to travel from end to end in the ampoule with each inversion.
4. Dry the ampoule and insert it into the photometer, flat end first, and obtain a reading.

NEW RE-TEST METHOD
As long as the SAM has not powered off, it is possible to “remix and re-read” a test ampoule at this point. To take a second reading, press and hold the ! key, then press the Zero/Test key. Release the ! key and Zero/Test key simultaneously. “O3” blinks on the display for a few seconds and then a new test result is displayed.

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