Williams Lab | Bates College | Instructional Video Series | May 2015
0:28 – Clean work area with RNase
0:48 – Create a plate map
1:04 – Step 1: Create a master mix
2:02 – Step 2: Add reactions to a 96-well plate
2:40 – Step 3: Cover reaction wells with optically clear caps
2:55 – Step 4: Spin the 96-well plate if necessary
3:15 – Run 96-well plate in the qPCR machine
![](https://s2.save4k.ru/pic/elQuA2vp6Fk/maxresdefault.jpg)