This Video is about Marek's Disease Vaccine ( HVT ) for Chickens & Marek's Disease in Poultry by Dr Arshad.
I will Share Marek's Disease Vaccine (HVT) Indications,
Warnings and cautions for Marek's Disease Vaccine , Direction and dosage information for Marek's Disease Vaccine.
Administer only as recommended.
Use entire contents when first opened.
Do not vaccinate within 21 days before slaughter.
Penicillin and Streptomycin Sulfate are added as bacteriostatic agents.
Contains Amphotericin B as a fungistatic agent.
Burn this container and all unused contents.
Storage Conditions
AMPULES: Store in liquid nitrogen container.
DILUENT: Store at room temperature.
LIQUID NITROGEN CONTAINER: Carefully observe all liquid nitrogen precautions including wearing eye protection and gloves. Store in a cool, well-ventilated area. Check liquid nitrogen level daily. Keep container away from incubator intakes and chicken boxes. CAUTION: DO NOT HOLD AMPULE TOWARD FACE WHEN REMOVING FROM A LIQUID NITROGEN CONTAINER. NEVER REFREEZE A VACCINE AMPULE AFTER THAWING.
PREPARATION OF VACCINE
1. Dilute the vaccine only as directed, observing all precautions and warnings for handling.
2. Remove from the liquid nitrogen only the ampules that are going to be used immediately. Move quickly, but carefully.
3. Place the ampule(s) in a large, clean container of water at 68° to 86°F (20° to 30°C) to thaw ampule quickly. Thaw the entire contents. Gently swirl the ampule to disperse contents. Break ampule at its neck and quickly proceed as described below.
4. Use 200 ml of sterile diluent for each 1,000 doses of vaccine to be injected subcutaneously or for each 4,000 doses of vaccine to be administered in ovo. Remove the cover from the diluent container. Draw contents of the ampule into a sterile 10 ml syringe fitted with an 18 to 20 gauge needle. Slowly add the contents of the vaccine ampule to the appropriate volume of diluent. Withdraw a small amount of the diluent, rinse the ampule once and add this to the vaccine-diluent mixture. Mix the contents of the diluent container thoroughly by swirling and inverting the container. Do not shake vigorously.
5. Keep the container of diluted vaccine in an ice bath. Use the vaccine-diluent mixture immediately. Agitate as needed to ensure a uniform suspension of the cells.
Use Of Vaccine For Subcutaneous Injection
IMPORTANT: Sterilize vaccinating equipment by autoclaving a minimum of 15 minutes at 250°F (121°C) or boiling in water for at least 20 minutes. Never allow chemical disinfectants to come in contact with vaccinating equipment.
1. Use a sterile automatic syringe with a 20 to 22 gauge 3/8” to 1/2” needle that is set to accurately deliver 0.2 ml per dose. Check the accuracy of delivery several times during the vaccination procedure.
2. Inject chickens under the loose skin at the back of the neck (subcutaneously), holding the chicken by the back of the neck just below the head. The loose skin in this area is raised by gently pinching with the thumb and forefinger. Insert the needle beneath the skin in a direction away from the head. Inject 0.2 ml per chicken. Avoid hitting the muscles and bones in the neck.
3. Use the entire contents of the vaccine container within one hour after mixing the vaccine with the diluent.
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