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Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate - a 2 minute Preview of the Experimental Protocol

Jean-Marc Taymans, Fangye Gao, Veerle Baekelandt
KU Leuven and Leuven Institute for Neuroscience and Disease (LIND), Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences;

Leucine rich repeat kinases 1 and 2 (LRRK1 and LRRK2) are multidomain proteins which encode both GTPase and kinase domains and which are phosphorylated in cells. Here, we present a protocol to label LRRK1 and LRRK2 in cells with 32P orthophosphate, thereby providing a means to measure their overall cellular phophorylation levels.

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