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Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue - a 2 minute Preview of the Experimental Protocol
Tami T. Tamashiro, Clifton Lee Dalgard, Kimberly R. Byrnes
Uniformed Services University, Neuroscience Program; Uniformed Services University, Department of Anatomy, Physiology, and Genetics; Uniformed Services University, Molecular and Cell Biology;
Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.
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