This video is about how to check the specificity of primers with the help of In Silico PCR. In Silico PCR is a tool in UCSC genome browser which is used to verify the primers.
How to design primers using Primer3Plus
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How to check the specificity of primers using NCBI primer blast
[ Ссылка ]
How to get sequence of any gene using UCSC genome browser
[ Ссылка ]
How to get sequence of any gene using NCBI GenBank
[ Ссылка ]
Principle and procedure of Polymerase Chain Reaction (PCR)
[ Ссылка ]
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