Fengzhu Xiong, Ian Swinburne, Sean Megason (Harvard Medical School)
Yolk syncytial layer(YSL) is an important structure in early zebrafish embryos that controls morphogenesis and regulate mesoderm/endoderm patterning. This movie provides a single-cell resolution side view of a developing zebrafish embryo from 16-cell stage to 5.5 hours post fertilization (hpf) which highlights the formation of the YSL. Movie is a continuous sequence of 160 images with time stamp (unit:hours and minutes post fertilization, EVL, enveloping layer) at 7 frame/second. Each image is a 3D projection of a Z-stack (251μm deep) of confocal slices (606μm in height and width, Scale bar: 50μm) at 1.5 minute time intervals. Cell membranes were labeled with membrane targeted yellow fluorescent protein (citrine, colored cyan in the movie) and cell nucleus with histone2B tagged red fluorescent protein (tdTomato, colored yellow in the movie). Both markers are from maternal deposited mRNAs and proteins initially then followed by zygotic expression. Laser line 514nm was used to excite both fluorescent proteins at the same time.

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