When Scrolling a paraffin tissues block it will be very hard to get a clean sample with out DNA contamination. Even with a totally scrubbed down microtome you cannot remove all DNA. It is impossible. We recommend trying your best to keep the scroll on the blade to minimize contamination. The blade will be the cleanest area on the microtome if the blade was not touched by human hands.
The Arraymold Tissue Microarray instrument company has been around for over 10 years. We pride ourselves in being the only company with free instructional videos for training technicians that include basic to advanced techniques on TMA construction. Our staff has been creating TMAs for almost 20 years, we are very experienced and are available to answer your questions so please email or call us if you have any issues punching or cutting a TMA.
Email: arraymold@gmail.com
We also recommend using needles with stylets instead of plungers. Especially for paraffin TMA construction. Plungers needles were never intended to be used for paraffin tissue blocks. Arraymold tested needle options over 10 years ago and found the plunger needles to be inadequate for TMA construction. That is why Arraymold is the only mold TMA instrument company on the market that doesn't use plunger needles.
For more information on Tissue Microarray construction please visit:
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