Variety of Active Organisms observed in a sample of active sludge treated waster water, This was collected from the round clarifier tank on a warm sunny day in May.
There are five major groups of microorganisms generally found in the aeration basin of the activated sludge process:
1. Bacteria-Aerobic bacteria remove organic nutrients
2. Protozoa-Remove & digests dispersed bacteria and suspended particles
3. Metazoa-Dominate longer age systems including lagoons
4. Filamentous bacteria-bulking sludge (poor settling & turbid effluent)
5. Algae and fungi-Fungi is present with pH changes & older sludge
1. Bacteria are primarily responsible for removing organic nutrients from the wastewater.
2. Protozoa play a critical role in the treatment process by removing and digesting free swimming dispersed bacteria and other suspended particles. This improves the clarity of the wastewater effluent. Like bacteria, some protozoa need oxygen, some require very little oxygen, and a few can survive without oxygen.
The types of protozoa present give us some indication of treatment system performance which are classified as follows:
1. Amoebae-Little effect on treatment & die off as amount of food decreases
2. Flagellates-Feed primarily on soluble organic nutrients
3. Ciliates-Clarify water by removing suspended bacteria
4. Ciliates; Free-swimming-Removes free-dispersed bacteria
5. Ciliates; Crawling (grazing)-Dominate activated sludge/good treatment
6. Ciliates; Stalked (sessile)-Dominates at process end
Protozoa images are below:
3. Metazoa are multi-cellular organisms which are larger than most protozoa and have very little to do with the removal of organic material from the wastewater. Although they do eat bacteria, they also feed on algae and protozoa. A dominance of metazoa is usually found in longer age systems; namely, lagoon treatment systems. Although their contribution in the activated sludge treatment system is small, their presence does indicate treatment system conditions.
Three most common metazoa found in the activated sludge treatment system.
1. Rotifers-Clarify effluent & are first affected by toxic loads
2. Nematodes-Feed on bacteria, fungi, small protozoa & other nematodes
3. Tardigrades (water bear)-Survive environmental extremes & toxic sensitivity
Metazoa images are below:
4. Filamentous bacteria are present when operational conditions drastically change. These bacteria grow in long filaments begin to gain an advantage. Changes in temperature, pH, DO, sludge age, or even the amounts of available nutrients such as nitrogen, phosphorus, oils & grease can affect these bacteria. The dominance of filamentous bacteria in the activated sludge treatment system can cause problems with sludge settling. At times excessive numbers of filamentous microorganisms interfere with floc settling and the sludge becomes bulky. This bulking sludge settles poorly and leaves behind a turbid effluent. Some filamentous microorganisms may cause foaming in the aeration basin and clarifiers.
Filamentous images are below:
5. Algae and fungi which are photosynthetic organisms and generally do not cause problems in activated sludge treatment systems, however there presence in the treatment system usually indicate problems associated pH changes and older sludge.
Fungi image is below:
Microorganisms in activated sludge has been described above, however if you have specific issues controlling microorganisms or other wastewater queries, please submit a question.
