This video explains how to do the following things in image J/Fiji
How to split fluoresce channels-0:13
Changing the image to grayscale-0:50
Merge the fluorescence channels into one image/composite image-1:17
Setting up a scale bar-1:46
Setting up a new scale bar-2:40
Measuring the diameter of a spherical mass-4:28
How to avoid repeated measurements-5:04
How to summarize measurements and export data to Excel sheet-5:10
How to duplicate an image, select an area of interest and measure the area of a spherical particle -7:05
Thresholding and analyzing particle size-8:16
How to analyze area if the particle/spherical edges are irregular-9:55
How to overlay a grid on an image using image J to calculate the percentage area of interest-11:46
How to remove the grid and save the image-13:34
