Formalin-fixed, 300 micron-thick mouse spleen section was blocked, permeabilized and stained overnight with the following:
Red: Alexa Fluor® 647 anti-mouse CD4 (clone RM4-5) - [ Ссылка ]
Green: Alexa Fluor® 488 anti-mouse CD169 (Siglec-1) (clone 3D6.112) - [ Ссылка ]
Blue: Alexa Fluor® 594 anti-mouse/human CD45R/B220 (clone RA3-6B2) - [ Ссылка ]
All antibodies were used at 5 µg/mL. Tissues were optically cleared, then analyzed at 220 µm imaging depth on a confocal microscope.
